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Biomineralization-inspired copper-cystine nanoleaves capable of laccase-like catalysis for the colorimetric

Miao Guan, Mengfan Wang, Wei Qi, Rongxin Su, Zhimin He

《化学科学与工程前沿（英文）》 2021年第15卷第2期页码 310-318 doi: 10.1007/s11705-020-1940-y

摘要： Recently, many efforts have been dedicated to creating enzyme-mimicking catalysts to replace natural enzymes in practical fields. Inspired by the pathological biomineralization behaviour of L-cystine, in this study, we constructed a laccase-like catalyst through the co-assembly of L-cystine with Cu ions. Structural analysis revealed that the formed catalytic Cu-cystine nanoleaves (Cu-Cys NLs) possess a Cu(I)-Cu(II) electron transfer system similar to that in natural laccase. Reaction kinetic studies demonstrated that the catalyst follows the typical Michaelis-Menten model. Compared with natural laccase, the Cu-Cys NLs exhibit superior stability during long-term incubation under extreme pH, high-temperature or high-salt conditions. Remarkably, the Cu-Cys NLs could be easily recovered and still maintained 76% of their activity after 8 cycles. Finally, this laccase mimic was employed to develop a colorimetric method for epinephrine detection, which achieved a wider linear range (9–455 μmol·L ) and lower limit of detection (2.7 μmol·L ). The Cu-Cys NLs also displayed excellent specificity and sensitivity towards epinephrine in a test based on urine samples.

关键词： biomineralization laccase L-cystine colorimetric detection enzyme mimic

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Cystine-assisted accumulation of gold nanoparticles on ZnO to construct a sensitive surface-enhanced

《化学科学与工程前沿（英文）》 2023年第17卷第1期页码 15-23 doi: 10.1007/s11705-022-2177-8

摘要： Recently, various semiconductor/metal composites have been developed to fabricate surface-enhanced Raman spectroscopy substrates. However, low metal loading on semiconductors is still a challenge. In this study, cystine was introduced to increase the accumulation of gold nanoparticles on zinc oxide, owing to the biomineralization property of cystine. Morphological analysis revealed that the obtained ZnO/Au/cystine composite not only had a higher metal loading but also formed a porous structure, which is beneficial for Raman performance. Compared with ZnO/Au, the ZnO/Au/cystine substrate displayed a 40-fold enhancement in the Raman signal and a lower limit of detection (10^–11 mol·L⁻¹) in the detection of rhodamine 6G. Moreover, the substrate has favorable homogeneity and stability. Finally, ZnO/Au/cystine displayed excellent performance toward crystal violet and methylene blue in a test based on river water samples. This study provided a promising method to fabricate sensitive semiconductor/noble metal-based surface-enhanced Raman spectroscopy substrates for Raman detection.

关键词： biomineralization cystine semiconductor/metal composite SERS detection Raman detection

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Cystine oligomers successfully attached to peptide cysteine-rich fibrils

Christian Bortolini,Mingdong Dong

《化学科学与工程前沿（英文）》 2016年第10卷第1期页码 99-102 doi: 10.1007/s11705-016-1554-6

摘要： Amyloid peptides are renowned to be related to neurodegenerative diseases, however, a fruitful avenue is to employ them as high-performance nanomaterials. These materials benefit from the intrinsic outstanding mechanical robustness of the amyloid backbone made of -strands. In this work, we exploited amyloid-like fibrils as functional material to attach pristine L-cysteine aggregates (cystine oligomers) and gold nanoparticles, without the need of templating compounds. This work will open new avenues on functional materials design and their realisation.

关键词： cysteine peptide fibrils gold nanoparticles amyloids oligomers nanomaterials